全文获取类型
收费全文 | 2108篇 |
免费 | 230篇 |
国内免费 | 25篇 |
专业分类
耳鼻咽喉 | 21篇 |
儿科学 | 83篇 |
妇产科学 | 19篇 |
基础医学 | 217篇 |
口腔科学 | 44篇 |
临床医学 | 282篇 |
内科学 | 540篇 |
皮肤病学 | 34篇 |
神经病学 | 112篇 |
特种医学 | 152篇 |
外科学 | 434篇 |
综合类 | 60篇 |
现状与发展 | 20篇 |
预防医学 | 101篇 |
眼科学 | 38篇 |
药学 | 83篇 |
中国医学 | 2篇 |
肿瘤学 | 121篇 |
出版年
2023年 | 47篇 |
2021年 | 31篇 |
2020年 | 38篇 |
2019年 | 20篇 |
2018年 | 70篇 |
2017年 | 58篇 |
2016年 | 53篇 |
2015年 | 60篇 |
2014年 | 62篇 |
2013年 | 100篇 |
2012年 | 62篇 |
2011年 | 76篇 |
2010年 | 73篇 |
2009年 | 92篇 |
2008年 | 78篇 |
2007年 | 73篇 |
2006年 | 101篇 |
2005年 | 90篇 |
2004年 | 74篇 |
2003年 | 79篇 |
2002年 | 88篇 |
2001年 | 80篇 |
2000年 | 78篇 |
1999年 | 63篇 |
1998年 | 60篇 |
1997年 | 55篇 |
1996年 | 61篇 |
1995年 | 38篇 |
1994年 | 25篇 |
1993年 | 31篇 |
1992年 | 30篇 |
1991年 | 27篇 |
1990年 | 25篇 |
1989年 | 23篇 |
1988年 | 23篇 |
1987年 | 27篇 |
1986年 | 20篇 |
1985年 | 23篇 |
1984年 | 13篇 |
1983年 | 12篇 |
1982年 | 12篇 |
1981年 | 9篇 |
1979年 | 16篇 |
1978年 | 20篇 |
1977年 | 14篇 |
1976年 | 12篇 |
1975年 | 11篇 |
1974年 | 11篇 |
1969年 | 12篇 |
1968年 | 11篇 |
排序方式: 共有2363条查询结果,搜索用时 46 毫秒
61.
62.
Total joint replacement has been one of the most remarkable successes of modern medical technology. Once John Charnley had
solved the problems of implant design, choice of materials, implant fixation, and infection (initial rates of infection were
approximately 10%), the way was clear for the widespread use of this valuable treatment, which is highly effective at removing
pain and restoring function. Unfortunately, infection still remains an important, though less common, problem. It is associated
with serious morbidity (pain, loss of function, wound breakdown, wound discharge, implant failure) and sometimes mortality.
It may be impossible to eradicate or suppress infection in the long term without removal of the prosthesis, and most clinicians
would consider it unwise to re-implant a new prosthesis in the presence of infection. Hence, patients with infected prosthetic
joints generally require multiple additional operations and prolonged periods of antibiotic therapy. Even radical attempts
at cure may fail (in 10% to 15% of cases in most series), requiring further cycles of treatment with progressively deteriorating
function. Thus, the treatment of prosthetic joint infection is arduous for the patient and the health care team, with no guarantee
of success. 相似文献
63.
Rau R 《Clinical rheumatology》2005,24(3):189-202
This review tries to answer the question of whether in the face of the recently introduced biologics conventional disease-modifying antirheumatic drugs (DMARDs) can still be recommended in the treatment of rheumatoid arthritis (RA). We start with an overview of the oldest conventional DMARD, injectable gold (Au), which was introduced in the treatment of RA in the 1920s. The effect of gold is directed at a number of different sites of the immune system. A significant improvement of clinical and biochemical disease activity parameters as well as an inhibition of X-ray progression has been shown in many studies. Head-to-head comparisons between gold and high-dose methotrexate (MTX) demonstrated no significant difference but some advantages for gold. Since trials comparing biologics with gold will never be performed, an indirect comparison was done by analyzing the results of trials with gold with those with biologics. Conclusions from such comparisons have to be drawn with caution especially since the methodology for performing trials has changed with time. We selected four trials with gold (two open, one placebo-controlled, and one comparison with MTX) and five trials with biologics (three placebo-controlled, one dose escalation study, and one comparison with MTX). In all these trials baseline data regarding swollen joint count (SJC), erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) were roughly comparable and, with the exception of interleukin (IL)-1 RA, demonstrated a similar improvement of over 50% already after 6 months [with faster onset with tumor necrosis factor (TNF)-alpha blockade]. American College of Rheumatology (ACR) response data were not available for the older gold trials. European League Against Rheumatism (EULAR) response criteria could be calculated for the Au/MTX trial and were—for these compounds—only slightly inferior to the results with adalimumab. X-ray response is especially difficult to compare across studies. Although an inhibition with Au and MTX could be demonstrated, this occurred—similar to corticosteroid treatment—earlier and more pronounced with TNF-alpha blockers. We confirm the statement of Weinblatt that the most modern DMARDs do not appear to be much better than the oldest one indicating that conventional DMARDs are not outdated. Therefore, a sufficient trial of conventional DMARDs should precede the introduction of treatment with the very expensive biologics. 相似文献
64.
65.
66.
NADPH oxidase is an enzyme in the plasma membrane of the neutrophil that catalyzes the production of O2-, a species central to the oxygen- dependent killing mechanisms of this cell. The oxidase is dormant in resting cells and is activated upon the addition of a stimulus. Neutrophils of patients with chronic granulomatous disease (CGD) manifest no oxidase activity when stimulated. The possible role of protein phosphorylation in the activation of NADPH oxidase was examined in normal and CGD neutrophils by measuring the incorporation of 32Pi into proteins as determined by gel electrophoresis followed by autoradiography. Resting neutrophils from normal subjects exhibit at least 40 distinct phosphoprotein bands. The level of phosphorylation of these bands was examined after the addition of phorbol myristate acetate (PMA), opsonized zymosan, digitonin, N-formyl-methionyl- phenylalanine (FMLP), or NaF. PMA and opsonized zymosan increased the phosphorylation of a set of 6 protein bands. Digitonin and FMLP consistently caused the phosphorylation of 4 of these protein bands, while NaF failed to induce increased phosphorylation of any protein band. All activators tested caused the dephosphorylation of one specific protein band. The time course of phosphorylation (dephosphorylation) was examined using PMA as the activating agent. Increased phosphorylation of one protein band was evident by 12 sec after the addition of PMA. The most slowly phosphorylated protein band did not slow evidence of change until 5 min after the addition of PMA. Three of the phosphoproteins examined were phosphorylated either earlier than or concomitant with the activation of NADPH oxidase. CGD neutrophils were compared with normal cells for their ability to phosphorylate proteins in response to PMA. The phosphoprotein banding patterns of CGD neutrophils were identical with those of normal neutrophils in both the resting and activated states. The evidence presented shows that the phosphorylation of proteins is a prominent feature of neutrophil metabolism. The striking similarity of phosphorylation changes induced by the various activators tested suggests that protein phosphorylation may play a role in some aspects of neutrophil activation. Evidence was not obtained, however, regarding a link between protein phosphorylation and activation of NADPH oxidase. 相似文献
67.
Osteomyelitis (bone infection) and neuro-osteoarthropathy (Charcot arthropathy) are limb-threatening complications of diabetic
neuropathy with very different therapies. Distinguishing between them may be difficult, but it is important. In Charcot arthropathy,
noninfectious soft tissue inflammation accompanies rapidly progressive destruction, first of joints, then of bone. This occurs
in a well-vascularized and severely neuropathic, but nonulcerated, foot. In osteomyelitis, chronic soft tissue ulceration
precedes infection of bone, which may be physically exposed. Magnetic resonance imaging and bone biopsy are the preferred
diagnostic tests, provided adequate technical and interpretive skills are available. 相似文献
68.
Repair of erosions in rheumatoid arthritis does occur. Results from 2 studies by the OMERACT Subcommittee on Healing of Erosions 总被引:4,自引:0,他引:4
Sharp JT Van Der Heijde D Boers M Boonen A Bruynesteyn K Emery P Genant HK Herborn G Jurik A Lassere M McQueen F Østergaard M Peterfy C Rau R Strand V Wassenberg S Weissman B;Subcommittee on Healing of Erosions of the OMERACT Imaging Committee 《The Journal of rheumatology》2003,30(5):1102-1107
The committee was charged with determining whether healing of erosions in rheumatoid arthritis (RA) occurs. Two exercises were performed: The first asked the committee members, as a panel of experts, to express agreement or disagreement with the presence of improvement and features of bone reaction to injury in images submitted by members as examples of healing. The second presented panel members with 28 pairs of serial images, 14 chosen to illustrate progression and 14 chosen to illustrate repair. Agreement was tested on 8 items: global judgment on which image in the pair was better, relative size of the erosion in the 2 images, judgment on which image was first, presence and extent of sclerosis, cortication, filling-in, remodeling, and reconstituting normal structure. Our results showed good agreement, among the 15 respondents, on global assessment of which image was better and which image showed the smaller erosion. Correct assignment of sequence was only slightly better than expected by chance (in 65% of the cases). Agreement was poor regarding the presence of morphologic features of bone repair. A majority of a panel of experts agreed on which 2nd images in a set of paired, serial images represented improvement and which showed progression based on global assessment of which was better and on size of erosion. Features of bone repair were not distinctive and did not enable the panel to deduce the correct sequence of the serial images. This study provides evidence that repair of bone damage in RA does occur, resulting in some degree of improvement, which was recognized by a majority of a panel of experts. 相似文献
69.
The role of defective fibrinolysis caused by elevated activity of plasminogen activator inhibitor-1 (PAI-1) in promoting fibrin deposition in vivo has not been well established. The present study compared the efficacy of thrombin or ancrod, a venom-derived enzyme that clots fibrinogen, to induce fibrin formation in rabbits with elevated PAI-1 levels. One set of male New Zealand rabbits received intravenous endotoxin to increase endogenous PAI-1 activity followed by a 1-hour infusion of ancrod or thrombin; another set of normal rabbits received intravenous human recombinant PAI-1 (rPAI-1) during an infusion of ancrod or thrombin. Thirty minutes after the end of the infusion, renal fibrin deposition was assessed by histopathology. Animals receiving endotoxin, rPAI-1, ancrod, or thrombin alone did not develop renal thrombi. All endotoxin-treated rabbits developed fibrin deposition when infused with ancrod (n = 4) or thrombin (n = 6). Fibrin deposition occurred in 7 of 7 rabbits receiving both rPAI-1 and ancrod and in only 1 of 6 receiving rPAI-1 and thrombin (P < .01). In vitro, thrombin but not ancrod was inactivated by normal rabbit plasma and by purified antithrombin III or thrombomodulin. The data indicate that elevated levels of PAI-1 promote fibrin deposition in rabbits infused with ancrod but not with thrombin. In endotoxin-treated rabbits, fibrin deposition that occurs with thrombin infusion may be caused by decreased inhibition of procoagulant activity and not increased PAI-1 activity. 相似文献
70.
Dubal DB Rau SW Shughrue PJ Zhu H Yu J Cashion AB Suzuki S Gerhold LM Bottner MB Dubal SB Merchanthaler I Kindy MS Wise PM 《Endocrinology》2006,147(6):3076-3084
Estradiol enhances plasticity and survival of the injured brain. Our previous work demonstrates that physiological levels of estradiol protect against cerebral ischemia in the young and aging brain through actions involving estrogen receptors (ERs) and alterations in gene expression. The major goal of this study was to establish mechanisms of neuroprotective actions induced by low levels of estradiol. We first examined effects of estradiol on the time-dependent evolution of ischemic brain injury. Because estradiol is known to influence apoptosis, we hypothesized that it acts to decrease the delayed phase of cell death observed after middle cerebral artery occlusion (MCAO). Furthermore, because ERs are pivotal to neuroprotection, we examined the temporal expression profiles of both ER subtypes, ERalpha and ERbeta, after MCAO and delineated potential roles for each receptor in estradiol-mediated neuroprotection. We quantified cell death in brains at various times after MCAO and analyzed ER expression by RT-PCR, in situ hybridization, and immunohistochemistry. We found that during the first 24 h, the mechanisms of estradiol-induced neuroprotection after MCAO are limited to attenuation of delayed cell death and do not influence immediate cell death. Furthermore, we discovered that ERs exhibit distinctly divergent profiles of expression over the evolution of injury, with ERalpha induction occurring early and ERbeta modulation occurring later. Finally, we provide evidence for a new and functional role for ERalpha in estradiol-mediated protection of the injured brain. These findings indicate that physiological levels of estradiol protect against delayed cell death after stroke-like injury through mechanisms requiring ERalpha. 相似文献